專任教師

吳意珣 (I-Son Ng) 教授

聯絡方式:

研究室:93712    
分機號碼:(06)-2757575轉62648
傳真:(06)-2344496
E-mail:yswu@mail.ncku.edu.tw
個人網頁:http://fungeneprotein.wordpress.com
研究專長:
酶與蛋白質工程、生質能源、基因工程、蛋白質體學、合成生物學


NCKU Research: https://researchoutput.ncku.edu.tw/en/persons/i-son-ng

個人CV :

學歷

  • 國立成功大學  化學工程研究所博士(中華民國 9109 - 9407
  • 國立成功大學  化學工程研究所碩士(中華民國 8509 - 8706
  • 國立成功大學  化學工程系學士(中華民國 8109 - 8506

經歷

  • 國立成功大學  化學工程系教授(中華民國 11008 ~now
  • 國立成功大學  化學工程系副教授(中華民國 10608 ~11007
  • 福建廈門大學  化學工程與生物工程系副教授(中華民國 9905 - 10307
  • 中央研究院南部生物技術中心 博士後研究員(中華民國 9508 - 9803
  • 台灣積體電路公司  製程工程師(中華民國 8907 - 9008
  • 比賽及得獎

  • 2021年國立成功大學工學院研究優良獎
  • 2021年國立成功大學大學創新與大學社會責任教學優良獎
  • 2021iGEM World Championship, Gold Prize, Virtual Jamboree, USA
  • 2020iGEM World Championship, Gold Prize and Best Hardware, Virtual Jamboree, USA
  • 2020年國立成功大學大學創新與大學社會責任教學優良獎
  • 2019iGEM World Championship, Grand Prize (1st winner), Gold Prize and Best Therapeutic Prize, Best Presentation, Best Hardware, Best Model, Best Measurement, Boston, USA
  • 2018iGEM World Championship, Gold Prize and Best Environmental Prize, Best Presentation, Boston, USA
  • 2018年龍騰微笑第一屆智聯網佳作
  • 2017iGEM World Championship, Gold Prize and Best Environmental Prize, Boston, USA
  • 2016iGEM World Championship, Gold Prize, Boston, USA
  • 2015年成大化工系學生輔導優良教師
  • 2014年台灣化學工程學會第61屆石延平教授論文獎

開授課程

  • 高等反應工程   (103 ~ )
  • 酵素與醱酵工程 (103 ~ )
  • 大一化工概論   (103 ~ 105)
  • 大四專題討論   (103 ~ 105)
  • 研究所專題討論 (106 ~ 109)
  • 能源概論       (103 ~ 104)
  • 合成生物學     (105 ~ 106大學部,107 ~ 研究所)
  • Chemical Engineering II (In English) (105 ~ )
  • 不分系跨領域學術專題: 遇見合成生物學上、下 (108~ )

教師著作

2022

  1. Jia-Yi Lin, Shih-I Tan, Ying-Chen Yi, Chuan-Chieh Hsiang, Chien-Hsiang Chang, Chun-Yen Chen, Jo-Shu Chang, I-Son Ng * (2022) High-level production and extraction of C-Phycocyanin from Cyanobacteria Synechococcus sp. PCC7002 for antioxidation, antibacterial and lead adsorption. Environmental Research, 206, 112283
  2. Tsung-Han Tsai, Jia-Yi Lin, I-Son Ng* (2022) Cooperation of phytoene synthase, pyridoxal kinase and carbonic anhydrase for enhancing carotenoids biosynthesis in genetic Chlamydomonas reinhardtii. Journal of the Taiwan Institute of Chemical Engineers, 104184
  3. Yu-Chieh Lin, Chengfeng Xue, Shih-I Tan, Wan-Wen Ting, Shih-Chen Yang, I-Son Ng* (2022) Precise measurement of decarboxylase and applied cascade enzyme for simultaneous cadaverine production with carbon dioxide recovery. Journal of the Taiwan Institute of Chemical Engineers, 104188
  4. Tzu-Hsuan Yu, Shih-I Tan, Ying-Chen Yi, Chengfeng Xue, Wan-Wen Ting, Jui-Jen Chang, I-Son Ng* (2022) New insight into the codon usage and medium optimization toward stable and high-level 5-aminolevulinic acid production in Escherichia coli. Biochemical Engineering Journal, 177, 108259.
  5. Chengfeng Xue, I-Son Ng* (2022) Sustainable production of 4-aminobutyric acid (GABA) and cultivation of Chlorella sorokiniana and Chlorella vulgaris as circular economy. Bioresource Technology, 343: 126089

 

2021

  1. Jia-Yi Lin#, Way-Rong Lin#, I-Son Ng* (2021) CRISPRa/i with Adaptive Single Guide Assisted Regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production. Biotechnology Journal, 20;e2100514.
  2. Ying-Chen Yi, Chengfeng Xue, I-Son Ng* (2021) Low carbon footprint production of high-end 5-aminolevulinic acid via integrative strain engineering and RuBisCo-equipped Escherichia coli. ACS Sustainable Chemistry & Engineering, 9(46): 15623–15633
  3. Ying-Chen Yi, I-Tai Shih, Tzu-Hsuan Yu, Yen-Ju Lee, I-Son Ng* (2021) Challenges and opportunities of bioprocessing 5-aminolevulinic acid using genetic and metabolic engineering: a critical review. Bioresources and Bioprocessing, 8: 13.
  4. Shih-I Tan, Chuan-Chieh Hsiang, I-Son Ng* (2021) Tailoring genetic elements of plasmid-driven T7 system for stable and robust one-step cloning and protein expression in broad Escherichia coli. ACS Synthetic Biology, 10(2), 412-421. doi.org/10.1021/acssynbio.1c00014
  5. Ya-Yun Chiang, Dillirani Nagarajana, Yung-Chung Lo, Chun-Yen Chen, I-Son Ng, Chien-Hsiang Chang, Duu-Jong Lee, Jo-Shu Chang*. (2021) Succinic acid fermentation with immobilized Actinobacillus succinogenes using hydrolysate of carbohydrate-rich microalgal biomass. Bioresource Technology, 342: 126014
  6. Jia-Yi Lin, I-Son Ng* (2021) Production, isolation and characterization of C-phycocyanin from a new halo-tolerant Cyanobacterium aponinum using seawater. Bioresource Technology, 342: 125946
  7. Sefli Sri Wahyu Effendi, Chengfeng Xue, Shih-I Tan, I-Son Ng* (2021) Whole-cell biocatalyst of recombinant tyrosine ammonia lyase with fusion protein and integrative chaperone in Escherichia coli for high-level p-Coumaric acid production. Journal of the Taiwan Institute of Chemical Engineers, 128; 64-72.
  8. Chengfeng Xue, Ying-Chen Yi and I-Son Ng* (2021) Migration of glutamate decarboxylase by cold treatment on whole-cell biocatalyst triggered activity for 4-aminobutyric acid production in engineering Escherichia coli. International Journal of Biological Macromolecules 190: 113–119.
  9. Shih-I Tan, I-Son Ng* (2021) Stepwise optimization of genetic RuBisCO-equipped Escherichia coli for low carbon-footprint protein and chemical production. Green Chemistry, 23, 4800-4813.
  10. Wan-Wen Ting, Chih-Yu Huang, Pong-Yee Wu, Shih-Fang Huang, Hung-Yi Lin, Sheng-Feng Li, Jo-Shu Chang, I-Son Ng* (2021) Whole-cell biocatalyst for cadaverine production using stable, constitutive and high expression of L-lysine decarboxylase in recombinant Escherichia coli W3110. Enzyme and Microbial Technology, 133: 109458.
  11. I-Tai Shih, Ying-Chen Yi, I-Son Ng* (2021) Plasmid-free system and modular design for efficient 5-aminolevulinic acid production by engineered Escherichia coli. Applied Biochemistry and Biotechnology 193:2858–2871. https://doi.org/10.1007/s12010-021-03571-3
  12. Chengfeng Xue, Tzu-Hsuan Yu, I-Son Ng* (2021) Engineering pyridoxal kinase PdxY-integrated Escherichia coli strain and optimization for high-level 5-aminolevulinic acid production. Journal of the Taiwan Institute of Chemical Engineers, 120: 49-58.
  13. Shih-I Tan, I-Son Ng* (2021) CRISPRi-mediated NIMPLY logic gate for fine-tuning the whole-cell sensing toward simple urine glucose detection. ACS Synthetic Biology, 10(2), 412-421. doi.org/10.1021/acssynbio.1c00014
  14. Yi-Jun Lan, Shih-I Tan, Shu-Yun Cheng, Wan-Wen Ting, Chengfeng Xue, Tzu-Han Lin, Ming-Zhi Cai, Po-Ting Chen**, I-Son Ng* (2021) Development of Escherichia coli Nissle 1917 derivative by CRISPR/Cas9 and application for gamma-aminobutyric acid (GABA) production in antibiotic-free system. Biochemical Engineering Journal, 168, 107952.
  15. Ying-Chen Yi, I-Son Ng* (2021) Redirection of metabolic flux in Shewanella oneidensis MR-1 by CRISPRi and modular design for 5-aminolevulinic acid production. Bioresources and Bioprocessing, 8: 13.
  16. Jia-Yi Lin, Chengfeng Xue, Shih-I Tan, I-Son Ng* (2021) Pyridoxal kinase PdxY mediated carbon dioxide assimilation to enhance the biomass in Chlamydomonas reinhardtii CC-400. Bioresource Technology, 322: 124530.
  17. Chengfeng Xue, Kai-Min Hsu, Chen-Yaw Chiu, Yu-Kaung Chang, I-Son Ng* (2021) Fabrication of bio-based polyamide 56 and antibacterial nanofiber membrane from cadaverine. Chemosphere, 266, 128967.
  18. Sefli Sri Wahyu Effendi, Shih-I Tan, Wan-Wen Ting, I-Son Ng* (2021) Genetic design of co-expressed Mesorhizobium loti carbonic anhydrase and chaperone GroELS to enhancing carbon dioxide sequestration. International Journal of Biological Macromolecules, 167: 326-334.
  19. Sefli Sri Wahyu Effendi, Shih-I Tan, Wan-Wen Ting, I-Son Ng* (2021) Enhanced recombinant Sulfurihydrogenibium yellowstonense carbonic anhydrase activity and thermostability by chaperone GroELS for carbon dioxide biomineralization. Chemosphere, 271, 128461.

 

2020

  1. Shih-I Tan, I-Son Ng* (2020) Design and optimization of bioreactor to boost carbon dioxide assimilation in RuBisCo-equipped Escherichia coli. Bioresource Technology, 314, 123785.
  2. Mehmooda Fayyaza, Kit Wayne Chew, Pau Loke Show, Tau Chuan Ling, I-Son Ng, Jo-Shu Chang. (2020) Genetic engineering of microalgae for enhanced biorefinery capabilities. Biotechnology Advances, 43:107554. DOI/10.1016/j.biotechadv.2020.107554
  3. Bing-Lan Liu, Chien Wei Ooi, I-Son Ng, Pau Loke Show, Kai-Jie Line, Yu-Kaung Chang* (2020) Effective purification of lysozyme from chicken egg white by tris (hydroxymethyl)aminomethane affinity nanofiber membrane. Food Chemistry, 327: 127038. DOI/10.1016/j.foodchem.2020.127038
  4. Sefli Sri Wahyu Effendi#, Shih-I Tan#, Chien-Hsiang Chang, Chun-Yen Chen, Jo-Shu Chang, I-Son Ng* (2020) Development and fabrication of disease resistance protein in recombinant Escherichia coli. Bioresources and Bioprocessing, 7: 57.
  5. Wan-Wen Ting, Shih-I Tan, I-Son Ng* (2020) Development of chromosome-based T7 RNA polymerase and orthogonal T7 promoter circuit in Escherichia coli W3110 as a cell factory. Bioresources and Bioprocessing, 7: 54.
  6. Chengfeng Xue, Kai-Min Hsu, Wan-Wen Ting, Shih-Fang Huang, Hung-Yi Lin, Sheng-Feng Li, Jo-Shu Chang, I-Son Ng* (2020) Efficient biotransformation of L-lysine into cadaverine by strengthening pyridoxal 5’-phosphate-dependent proteins in Escherichia coli with cold shock treatment. Biochemical Engineering Journal, 161, 107659. DOI/10.1016/j.bej.2020.107659
  7. Wan-Wen Ting, I-Son Ng* (2020) Metabolic manipulation through CRISPRi and gene deletion to enhance cadaverine production in Escherichia coli. Journal of Bioscience and Bioengineering, 130(6), 553-562.
  8. I-Son Ng, ChienWei Ooi, Bing-Lan Liu, Chun-To Peng, Chen-Yaw Chiu, Yu-Kaung Chang⁎ (2020) Antibacterial efficacy of chitosan- and poly(hexamethylene biguanide)-immobilized nanofiber membrane. International Journal of Biological Macromolecules, 154: 844-854.
  9. Po-Ting Chen, Zih-Syuan Hong, Chieh-Lun Cheng, I-Son Ng, Yung-Chung Lo, Dillirani Nagarajan, Jo-Shu Chang* (2020) Exploring fermentation strategies for enhanced lactic acid production with polyvinyl alcohol-immobilized Lactobacillus plantarum 23 using microalgae as feedstock. Bioresource Technology, 308, 123266.
  10. Shih-I Tan, Peng-Jui Yu, I-Son Ng* (2020) CRISPRi-mediated programming essential gene can as a direct enzymatic performance evaluation & determination (DEPEND) system. Biotechnology and Bioengineering, 117:2842–2851.
  11. Shih-I Tan, I-Son Ng* (2020) New insight into plasmid-driven T7 RNA polymerase (PDT7) in Escherichia coli and used as genetic amplifier for biosensor. ACS Synthetic Biology, 9, 613−622. dx.doi.org/10.1021/acssynbio.9b00466
  12. Ying-Chen Yi, I-Son Ng * (2020) Establishment of toolkit and T7RNA polymerase/promoter system in Shewanella oneidensis MR-1. Journal of the Taiwan Institute of Chemical Engineers, 109: 8-14.
  13. I-Son Ng*, Batuhan Birol Keskin, Shih-I Tan (2020) A critical review of genome editing and synthetic biology applications in metabolic engineering of microalgae and cyanobacteria. Biotechnology Journal, e1900228. doi: 10.1002/biot.201900228.
  14. Yoong Kit Leong, Chien-Heng Chen, Shih-Fang Huang, Hung-Yi Lin, Sheng-Feng Li, I-Son Ng, Jo-Shu Chang* (2020) High-level L-lysine bioconversion into cadaverine with enhanced productivity using engineered Escherichia coli whole-cell biocatalyst. Biochemical Engineering Journal, 157, 107547.
  15. Chih-Yu Huang, Wan-Wen Ting, Ying-Chun Chen, Pong-Yee Wu, Cheng-Di Dong, Shih-Fang Huang, Hung-Yi Lin, Sheng-Feng Li, I-Son Ng*, Jo-Shu Chang** (2020) Facilitating the enzymatic conversion of lysine to cadaverine in engineered Escherichia coli with metabolic regulation by genes deletion. Biochemical Engineering Journal, 156, 107514.
  16. Way-Rong Lin, I-Son Ng* (2020) Development of CRISPR/Cas9 system in Chlorella vulgaris FSP-E to enhance lipid accumulation. Enzyme and Microbial Technology, 133: 109458.
  17. Shih-I Tan, Shao-Chun You, I-Tai Shih, I-Son Ng* (2020) Quantification, regulation and production of 5-aminolevulinic acid by green fluorescent protein in recombinant Escherichia coli. Journal of Bioscience and Bioengineering, 129 (4): 387-394.
  18. Sefli Sri Wahyu Effendi, Chen-Yaw Chiu, Yu-Kaung Chang, I-Son Ng* (2020) Crosslinked on novel nanofibers with thermophilic carbonic anhydrase for carbon dioxide sequestration. International Journal of Biological Macromolecules, 152 930–938. doi.org/10.1016/j.ijbiomac.2019.11.234
  19. Tzu-Hsuan Yu, Ying-Chen Yi, I-Tai Shih, I-Son Ng* (2020) Enhanced 5-aminolevulinic acid production by co-expression of codon optimized hemA gene with chaperone in genetic engineered Escherichia coli. Applied Biochemistry and Biotechnology, 191, 299-312. doi.org/10.1007/s12010-019-03178-9

 

2019

  1. Sefli Sri Wahyu Effendi, I-Son Ng* (2019) The prospective and potential of carbonic anhydrase for carbon dioxide sequestration: a critical review. Process Biochemistry, 87: 55-65. doi.org/10.1016/j.procbio.2019.08.018
  2. Way-Rong Lin, Shih-I Tan, Chuan-Chieh Hsiang, Po-Kuei Sung, I-Son Ng* (2019) Challenges and opportunity of recent genome editing and multi-omics in cyanobacteria and microalgae for biorefinery. Bioresource Technology, 291, 121932.
  3. Yu-Cheng Lai, Chien-Hsiang Chang, Chun-Yen Chen, Jo-Shu Chang, I-Son Ng* (2019) Towards protein production and application by using Chlorella species as circular economy. Bioresource Technology, 289, 121625.
  4. Bo Chuan Huang, Ying-Chen Yi, Jo-Shu Chang, I-Son Ng* (2019) Mechanism study of photo-induced gold nanoparticles formation by Shewanella oneidensis MR-1. Scientific Reports, 9:7589 
  5. Kao-Pang Hsu, Shih-I Tan, Chen-Yaw Chiu, Yu-Kaung Chang, I-Son Ng* (2019) ARduino-pH tracker (ART) and screening platform for characterization of recombinant carbonic anhydrase in Escherichia coli. Biotechnology Progress, DOI: 10.1002/btpr.2834
  6. Keju Jing, Yanlan Guo, I-Son Ng* (2019) Reveals Antigen 43 mediated surface display in Escherichia coli by different fusion sites and proteins. Bioresources and Bioprocessing, 6:14.
  7. I-Son Ng, Cher Pin Song, Chien Wei Ooi, Beng Ti Tey, Yu-Han Lee, Yu-Kaung Chang (2019) Purification of lysozyme from chicken egg white using nanofiber membrane immobilized with Reactive Orange 4 dye. International Journal of Biological Macromolecules, 134 (1): 458-468.
  8. I-Son Ng, Malcolm S.Y. Tang, Pau Loke Show, Zih-Ming Chiou, Jung-Chin Tsai, Yu-Kaung Chang* (2019) Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane. International Journal of Biological Macromolecules 132, 615–628.
  9. Yu-Kaung Chang*, Chien-Wei Ooi, Poh-En Liew, I-Son Ng, Yung-Nien Huang, Ze-Hong Chen (2019) Optimization of three-phase fluidized bed cell disruptor for the release of alcohol dehydrogenase from baker’s yeast. Chemical Engineering Journal, 386: 121224. DOI: 10.1016/j.cej.2019.03.100
  10. Jih-Heng Chen, Chun-Yen Chen, Tomohisa Hasunuma, Akihiko Kondo, Chien-Hsiang Chang, I-Son Ng, Jo-Shu Chang (2019) Enhancing lutein production with mixotrophic cultivation of Chlorella sorokiniana MB-1-M12 using different bioprocess operation strategies. Bioresource Technology, 278, 17–25.
  11. Wai Yan Cheah, Pau-Loke Show, I-Son Ng, Guan-Yu Lin, Chen-Yaw Chiu, Yu-Kaung Chang* (2019) Antibacterial activity of quaternized chitosan modified nanofiber membrane. International Journal of Biological Macromolecules, 126: 569–577.

 

2018

  1. Way-Rong Lin#, Yu-Cheng Lai#, Po-Kuei Sung, Shih-I Tan, Chien-Hsiang Chang, Chun-Yen Chen, Jo-Shu Chang, I-Son Ng* (2018) Enhancing carbon capture and lipid accumulation by genetic carbonic anhydrase in microalgae. Journal of the Taiwan Institute of Chemical Engineers, 93: 131–141.
  2. Si-Yu Li, I-Son Ng, Po Ting Chen, Chung-Jen Chiang, Yun-Peng Chao* (2018) Biorefining of protein waste for production of sustainable fuels and chemicals. Biotechnol Biofuels, 11:256.
  3. Shih-I Tan#, Yin-Lung Han#, You-Jin Yu, Chen-Yaw Chiu, Yu-Kaung Chang, Shoung Ouyang, Kai-Chun Fan, Kuei-Ho Lo, I-Son Ng* (2018) Efficient carbon dioxide sequestration by using recombinant carbonic anhydrase. Process Biochemistry, 73: 38-46.
  4. You-Jin Yu#, Zhiyang Chen#, Po Ting Chen, I-Son Ng* (2018) Production, characterization and antibacterial activity of exopolysaccharide from a new isolated Weissella cibaria under sucrose effect. Journal of Bioscience and Bioengineering, 126(6), 769-777.
  5. I-Son Ng*, Yanlan Guo#, Yunli Zhou#, Jhe-Wei Wu, Shih-I Tan, Ying-Chen Yi (2018) Turn on the Mtr pathway genes under pLacI promoter in Shewanella oneidensis MR-1. Bioresources and Bioprocessing, 5:35. doi.org/10.1186/s40643-018-0221-9
  6. Chun-Yen Chen, I-Chia Lu, Dillirani Nagarajan, Chien-Hsiang Chang, I-Son Ng, Duu-Jong Lee, Jo-Shu Chang*. (2018) A highly efficient two-stage cultivation strategy for lutein production using heterotrophic culture of Chlorella sorokiniana MB-1-M12. Bioresource Technology, 253, 141-147.

 

2017

  1. I-Son Ng*, You-Jin Yu, Ying-Chen Yi, Shih-I Tan, Bo-Chuan Huang, Yin-Lung Han (2017) Identification of gold sensing peptide by integrative proteomics and a bacterial two-component system. Frontiers in Chemistry, doi: 10.3389/fchem.2017.00127.
  2. Jhe-Wei Wu, I-Son Ng* (2017) Biofabrication of gold nanoparticles by Shewanella species. Bioresources and Bioprocessing, 4:50.
  3. I-Son Ng*, Chung-Chuan Hsueh, Bor-Yann Chen (2017) Electron transport phenomena of electroactive bacteria in microbial fuel cells: a review of Proteus hauseri. Bioresource and Bioprocessing, 4:53.
  4. I-Son Ng*, Shih-I Tan, Pei-Hsun Kao, Yu-Kaung Chang, Jo-Shu Chang. (2017) Recent developments on genetic engineering of microalgae for biofuels and bio-based chemicals. Biotechnology Journal, 12, 1600644.
  5. Chun-Yen Chen, Ai-Ling Kao, Zheng-Chia Tsai, Yu-Mei Shen, Pei-Hsun Kao, I-Son Ng*, and Jo-Shu Chang** (2017) Expression of synthetic phytoene synthase gene to enhance beta-carotene production in Scenedesmus sp. CPC2. Biotechnology Journal, doi: 10.1002/biot.201700204.
  6. Pei-Hsun Kao, I-Son Ng* (2017) CRISPRi mediated phosphoenolpyruvate carboxylase regulation to enhance the production of lipid in Chlamydomonas reinhardtii. Bioresource Technology, 245:1527–1537.
  7. Shih-I Tan, ISon Ng*, YouJin Yu (2017) Heterologous expression of an acidophilic multicopper oxidase in Escherichia coli and its applications in biorecovery of gold. Bioresources and Bioprocessing, 4:20.
  8. Chang Su, Zhiyang Chen, I-Son Ng* (2017) Impact of pH regulation on multi-copper oxidase production and swarming motility in the bacterium Proteus hauseri ZMd44. Biotechnology and Applied Biochemistry, DOI: 10.1002/bab.1556.
  9. I-Son Ng*, Chengfeng Xue. (2017) Enhanced exopolysaccharide production and biological activity of Lactobacillus rhamnosus ZY with calcium and hydrogen peroxide. Process Biochemistry, 53:295-304.

 

2016

  1. I-Son Ng*, Ying-Hsin Hung, Pei-Hsun Kao, Yunli Zhou, Xia Zhang. (2016) CRISPR/Cas9 nuclease cleavage enables marker-free genome editing in Escherichia coli: A sequential study. Journal of the Taiwan Institute of Chemical Engineers, 68: 31-39.
  2. Mu-En Chung, I-Hsin Yeh, Li-Yu Sung, Meng-Ying Wu, Yun-Peng Chao, I-Son Ng, Yu-Chen Hu* (2016) Enhanced Integration of Large DNA into E. coli Chromosome by CRISPR/Cas9. Biotechnology and Bioengineering 114: 172-183.
  3. Nan Wang, Xin Guo, I-Son Ng* (2016) Simultaneous release of recombinant cellulases introduced by coexpressing colicin E7 lysis in Escherichia coli. Biotechnology and Bioprocess Engineering, 21:491-501.
  4. Keju Jing*, Shuya He, Tingting Chen, Yinghua Lu, I-Son Ng* (2016) Enhancing beta-carotene biosynthesis and gene transcriptional regulation in Blakeslea trispora with sodium acetate. Biochemical Engineering Journal, 114:10-17.
  5. Theoneste Ndikubwimana, Jingyu Chang, Zongyuan Xiao, Wenyao Shao, Xianhai Zeng* I-Son Ng, Yinghua Lu* (2016) Flotation: A promising microalgae harvesting and dewatering technology for biofuels production. Biotechnology Journal, 11: 315-326.
  6. Yunli Zhou, I-Son Ng* (2016) Explored a cryptic plasmid pSXM33 from marine bacterium Shewanella xiamenensis BC01 and construction as the shuttle vector. Biotechnology and Bioprocess Engineering, 21:68-78.
  7. Xia Zhang, I-Son Ng*, Jo-Shu Chang (2016) Cloning, expression and characterization of a robust recombinant azoreductase from Shewanella xiamenensis BC01. Journal of the Taiwan Institute of Chemical Engineers, 61:97-105.
  8. I-Son Ng*, Chiming Ye, Yuzhe Li, Bor-Yann Chen (2016) Insights into copper effect on Proteus hauseri through proteomic and metabolic analyses. Journal of Bioscience and Bioengineering, 121(2) 178-185.

 

2015

  1. Xueping Ling*, Jing Guo, Chuqiang Zheng, Chiming Ye, Yinghua Lu, Xueshan Pan, Zhengqi Chen, I-Son Ng** (2015) Simple, effective protein extraction method and proteomics analysis from polyunsaturated fatty acids-producing micro-organisms. Bioprocess and Biosystems Engineering, 38: 2331-2341.
  2. I-Son Ng*, Ndive Chukwuma Isaac, Yunli Zhou, Xiaomin Wu (2015) Cultural optimization and metal effects of Shewanella xiamenensis BC01 growth and swarming motility. Bioresources and Bioprocessing, 2:28.
  3. Tingting Chen, Yunli Zhou, I-Son Ng*, Chun-Shan Yang, Hsiang-Yu Wang (2015) Formation and characterization of extracellular polymeric substance from Shewanella xiamenensis BC01 under calcium stimulation. Journal of the Taiwan Institute of Chemical Engineers, 57:175-181.
  4. Xueping Ling, Jing Guo, Xiaoting Liu, Xia Zhang, Nan Wang, Yinghua Lu, I-Son Ng* (2015) Impact of carbon and nitrogen feeding strategy on high production of biomass and docosahexaenoic acid (DHA) by Schizochytrium sp. LU310. Bioresource Technology, 184:139-147.
  5. I-Son Ng*, Fangxin Xu, Xia Zhang, Chiming Ye (2015) Enzymatic exploration of catalase from a nanoparticle producing and biodecolorizing algae Shewanella xiamenensis BC01 Bioresource Technology, 184:429-435.

 

2014

  1. Yuzhe Li, I-Son Ng*, Xia Zhang, Nan Wang (2014) Draft genome sequence of the dye-decolorizing and nanowire-producing bacterium Shewanella xiamenensis BC01. Genome announcement, 2(4). pii: e00721-14.
  2. I-Son Ng*, Xiaomin Wu, Yinghua Lu, Chuanyi Yao (2014) Trichoderma reesei cellulase complex in hydrolysis of agricultural waste of grapefruit peel and orange peel. BioResources, 9(4), 6420-6431.
  3. I-Son Ng*, Xuesong Zheng, Nan Wang, Bor-Yann Chen, Xia Zhang, Yinghua Lu (2014) Copper Response of Proteus hauseri Based on Proteomic and Genetic Expression and Cell Morphology Analyses. Applied Biochemistry and Biotechnology, 173:1057-1072.
  4. Yu Zhang, I-Son Ng*, Chuanyi Yao, Yinghua Lu. (2014) An orthogonal array deciphering MRS medium requirements for an isolated Lactobacillus rhamnosus ZY with cell properties characterization. Journal of Bioscience and Bioengineering, 118(3): 298-304.
  5. You-Ping Xie, Shih-Hsin Ho, Chun-Yen Chen, Ching-Nen Nathan Chen, Chen-Chun Liu, I-Son Ng, Ke-Ju Jing, Sheng-Chung Yang, Chi-Hui Chen, Jo-Shu Chang*, Ying-Hua Lu** (2014) Simultaneous enhancement of CO2 fixation and lutein production with thermo-tolerant Desmodesmus sp. F51 using a repeated fed-batch cultivation strategy. Biochemical Engineering Journal, 86:33-40.
  6. Youping Xie, Hang Li, Xiaowei Wang, I-Son Ng, Yinghua Lu*, Keju Jing* (2014) Kinetic Simulating of Cr (VI) Removal by the Waste Chlorella vulgaris Biomass. Journal of the Taiwan Institute of Chemical Engineers, 45: 1773-1782.
  7. Nan Wang, I-Son Ng*, Po Ting Chen**, Yi-Chung Chen, Bor-Yann Chen, Yinghua Lu (2014) Draft genome sequence of the bioelectricity-generating and dye-decolorizing bacterium Proteus hauseri strain ZMd44. Genome announcement, 2(1). pii: e00992-13.
  8. Chiming Ye, I-Son Ng*, Keju Jing, Yinghua Lu (2014) Direct proteomic mapping for Streptomyces roseosporus NRRL11379 with precursor and insights into daptomycin biosynthesis. Journal of Bioscience and Bioengineering, 117(5):591-597.
  9. I-Son Ng*, Tingting Chen, Rong Lin, Xia Zhang, Chao Ni, Dongzhe Shun (2014) Decolorization of textile azo dye and Congo red by an isolated strain of the dissimilatory manganese-reducing bacterium Shewanella xiamenensis BC01. Applied Microbiology and Biotechnology, 98(5):2297-2308.
  10. I-Son Ng*, Fangxin Xu, Chiming Ye, Bor-Yann Chen*, Yinghua Lu (2014) Exploring metal effects and synergistic interactions of ferric stimulation on azo-dye decolorization by new indigenous Acinetobacter guillouiae Ax-9 and Rahnella aquatilis DX2b. Bioprocess and Biosystems Engineering, 37(2): 217-224.
  11. I-Son Ng*, Chiming Ye, Zhixiang Zhang, Yinghua Lu, Keju Jing* (2014) Daptomycin antibiotic production processes in fed-batch fermentation by Streptomyces roseosporus NRRL11379 with precursor effect and medium optimization. Bioprocess and Biosystems Engineering, 37(3):415-423.

 

2013 and before

  1. Youping Xie, Shih-Hsin Ho, Ching-Nen Nathan Chen, Chun-Yen Chen, I-Son Ng, Ke-Ju Jing, Jo-Shu Chang, Yinghua Lu (2013) Phototrophic cultivation of a thermo-tolerant Desmodesmus sp. for lutein production: Effects of nitrate concentration, light intensity and fed-batch operation. Bioresource Technology 144:435–444.
  2. Bor-Yann Chen*, Chung-Chuan Hsueh, Shi-Qi Liu, I-Son Ng, Yu-Min Wang (2013) Deciphering mediating characteristics of decolorized intermediates for reductive decolorization and bioelectricity generation. Bioresource Technology, 145: 321-325.
  3. Bor-Yann Chen*, Junming Hong, I-Son Ng, Yu-Min Wang, Shi-Qi Liu, Bing Lin, Chao Ni (2013) Deciphering simultaneous bioelectricity generation and reductive decolorization using mixed-culture microbial fuel cells in salty media. Journal of the Taiwan Institute of Chemical Engineers 44: 446–453.
  4. I-Son Ng*, Xiaomin Wu, Xuemei Yang, Youping Xie, Yinghua Lu, Cuixue Chen (2013) Synergistic effect of Trichoderma reesei cellulases on agricultural tea waste for adsorption of heavy metal Cr(VI). Bioresource Technology, 145:297-301.
  5. I-Son Ng*, Xiaoqin Chi, Xiaomin Wu, Ziwei Bao, Yinghua Lu, Jo-Shu Chang, Xueping Ling** (2013) Cloning and expression of Cel8A from Klebsiella pneumoniae in Escherichia coli and comparison to cel gene of Cellulomonas uda. Biochemical Engineering Journal, 78: 53-58.
  6. I-Son Ng*, Xia Zhang, Yu Zhang, Yinghua Lu (2013) Molecular cloning and heterologous expression of laccase from Aeromonas hydrophila NIU01 in Escherichia coli with parameters optimization in production. Applied Biochemistry and Biotechnology, 169(7): 2223-2235.
  7. I-Son Ng*, Xuesong Zheng, Bor-Yann Chen, Xiaoqin Chi, Yinghua Lu, Chun-Sun Chang (2013) Proteomics approach to decipher novel genes and enzymes characterization of a bioelectricity-generating and dye-decolorizing bacterium Proteus hauseri ZMd44. Biotechnology and bioprocess engineering, 18, 1: 8-17.
  8. Xuesong Zheng, I-Son Ng*, Chiming Ye, Bor-Yann Chen, Yinghua Lu (2013) Copper ion-stimulated McoA-laccase Production and Enzyme Characterization in Proteus hauseri ZMd44. Journal of Bioscience and Bioengineering, 115, 388-393.
  9. Xianhai Zeng, Michael K. Danquah, Shiduo Zhang, Xia Zhang, Mengyang Wu, Xiao Dong Chen, I-Son Ng, Keju Jing, Yinghua Lu* (2012) Autotrophic cultivation of Spirulina platensis for CO2 fixation and phycocyanin production. Chemical Engineering Journal, 183, 192-197.
  10. Bor-Yann Chen*, Yu-Min Wang, I-Son Ng, Shi-Qi Liu, Jhao-Yin Hung (2012) Deciphering simultaneous bioelectricity generation and dye decolorization using Proteus hauseri. Journal of Bioscience and Bioengineering, 113, 502-507.
  11. Jing-Long Han, I-Son Ng, Yanni Wang, Xuesong Zheng, Wen-Ming Chen, Chung-Chuan Hsueh, Shi-Qi Liu, Bor-Yann Chen* (2012) Exploring new strains of dye-decolorizing bacteria. Journal of Bioscience and Bioengineering, 113, 508-514.
  12. Wei Li, I-Son Ng, Baishan Fang*, Jincong Yu, Guangya Zhang (2011) Codon optimization of 1,3-propanediol oxidoreductase expression in Escherichia coli and enzymatic properties. Electronic Journal of Biotechnology, DOI:10.2225/vol14-issue4-fulltext-9.
  13. Wei Li, I-Son Ng, Baishan Fang*, Guangya Zhang (2011) Expression and non-chromatographic production of 1,3-propanediol oxidoreductase in Escherichia coli. Electronic Journal of Biotechnology, DOI:10.2225/vol14-issue6-fulltext-2.
  14. I-Son Ng, Shau-Wei Tsai, Yu-Ming Ju, Su-May Yu*, Tuan-hua David Ho* (2011) Dynamic synergistic effect on Trichoderma reesei cellulases by novel beta-glucosidases from Taiwanese fungi. Bioresource Technology, 102: 6073-6081.
  15. Bor-Yann Chen*, Yu-Min Wang, I-Son Ng (2011) Understanding interactive characteristics of bioelectricity generation and reductive decolorization using Proteus hauseri. Bioresource Technology, 102(2), 1159-1165.
  16. I-Son Ng*, Po Ting Chen, Yu-Ming Ju, Shau-Wei Tsai (2010) Novel cellulase screening and optimal production from the wood decaying Xylariaceae: Daldinia species. Applied Biochemistry and Biotechnology, DOI 10.1007/s12010-010-9102-1.
  17. I-Son Ng, Chen-Wei Li, Shuang-Pi Chan, Jiun-Ly Chir, Po Ting Chen, Chii-Gong Tong, Su-May Yu*, Tuan-hua David Ho* (2010) High-level production of a thermoacidophilic b-glucosidase from Penicillium citrinum YS40-5 by solid-state fermentation with rice bran. Bioresource Technology, 101:1310-1317.
  18. I-Son Ng, Chen-Wei Li, Yi-Fang Yeh, Po Ting Chen, Jiun-Ly Chir, Chin-Hua Ma, Su-May Yu, Tuan-Hua David Ho*, Chii-Gong Tong* (2009) A novel endoglucanase from the thermophilic bacterium Geobacillus sp. 70PC53 with high activity and stability over a broad range of temperatures. Extremophiles, 13: 425-435.
  19. Shau-Wei Tsai*, Chun-Chi Chen, Hung-Shien Yang, I-Son Ng, The-Liang Chen (2006) Implication of substrate-assisted catalysis on improving lipase activity or enantioselectivity in organic solvents. BBA Proteins and Proteomics, 1764: 1424-1428.
  20. I-Son Ng, Shau-Wei Tsai* (2006) Investigation of lipases from various Carica papaya varieties for hydrolysis of olive oil and kinetic resolution of (R,S)-profen 2,2,2-trifluoroethyl thioesters. Process Biochemistry, 41: 540-546.
  21. I-Son Ng, Shau-Wei Tsai* (2006) Partially purified Carica papaya lipase: characteristics and application to the dynamic kinetic resolution of (R,S)-naproxen 2,2,2-trifluoroethyl thioester. Journal of the Taiwan Institute of Chemical Engineers, 37: 375-392.
  22. Wen-Yen Wen, I-Son Ng, Shau-Wei Tsai* (2006) Lipase-catalyzed dynamic hydrolytic resolution of (R,S)-2,2,2-trifluoroethyl a-chlorophenyl acetate in water-saturated isooctane. Journal of Chemical Technology and Biotechnology, 81: 1715-1721.
  23. I-Son Ng, Shau-Wei Tsai* (2005) Hydrolytic resolution of (R,S)-naproxen 2,2,2-trifluoroethyl thioester by Carica papaya lipase in water-saturated organic solvents. Biotechnology and Bioengineering 89:88-95.
  24. I-Son Ng, Shau-Wei Tsai* (2005) Partially purified Carica papaya lipase: a versatile biocatalyst for the hydrolytic resolution of (R,S)-2-arylpropionic thioesters in water-saturated organic solvents. Biotechnology and Bioengineering, 91:106-113.
  25. I-Son Ng, Shau-Wei Tsai* (2005) Effect of Carica papaya lipase pretreatments on enantioselective hydrolysis of (R,S)-2-arylpropionic thioesters. Journal of the Taiwan Institute of Chemical Engineers, 36: 331-337.
  26. Wei-Fu Hsu, I-Son Ng, Shau-Wei Tsai* (2003) Purification and characterization of creatinase by recombinant Escherichia coli. Journal of the Taiwan Institute of Chemical Engineers, 34: 305-310.
  27.  I-Son Ng, Shau-Wei Tsai*, Shu-Jen Chen (1999) Purification and characterization of extracellular lipase from Acinetobacter radioresistens CMC-2. Journal of the Taiwan Institute of Chemical Engineers, 30: 355-362.

研究

  • 定向進化與同步釋放重組纖維素酶的研究,科技部個別型計畫 (103101日至1050731)
  • 應用常間迴文重複序列-關聯蛋白系統(CRISPR/Cas)提昇微藻固碳效能,科技部個別型計畫 (10481日至1050731)
  • 應用合成生物學提昇希瓦氏菌產電及催化效能,科技部個別型計畫 (10581日至1080731)
  • 利用定向進化增強碳酸酐酶封存二氧化碳,科技部個別型計畫 (10581日至1080731)
  • 微藻生物質的開發與高值化應用,科技部非糧食生質資源計畫 (10581日至1060731)
  • 利用基因改造藻類生產化學品 (NEA041F001),中油綠能所 (10522 1051130)
  • 開發微藻碳循環材料高值化關鍵技術(1/3),科技部計畫 (10661日至1070531)
  • 開發微藻碳循環材料高值化關鍵技術(2/3),科技部計畫 (10761日至1080531)
  • 開發微藻碳循環材料高值化關鍵技術(3/3),科技部計畫 (10861日至1090531)
  • 循環經濟之二氧化碳利用與高值化技術開發,科技部計畫 (10881日至1090731)
  • 智能細胞工廠生產前驅藥物五胺基酮戊酸,科技部個別型計畫 (10881日至1110731)
  • 抗酸性基因調控生產高值化4-胺基丁酸(GABA)之機制與應用,科技部個別型計畫 (11081日至1130731)
  • 生質衣康酸重組微生物菌株開發與小量生產,中石化產學合作計畫 (11011日至1101231)
  • 生質衣康酸重組微生物菌株開發及生產純化,中石化產學合作計畫 (11111日至1111231)

服務

  • 108 研究生委員會
  • 103104105106107108109  儀器委員會
  • 103106107109  國際事務委員會
  • 104105 學生事務委員會
  • 108 ~ 迄今   台灣生物技術與生化工程學會 理事
  • 108/08       Applied Biochemistry and Biotechnology, Guest Editor of Special Issue
  • 105 ~ 107    台灣生物技術與生化工程學會 監事
  • 104 ~ 迄今   Bioresources and Bioprocessing, 副編輯
  • 成大iGEM跨領域學術團隊創隊指導教授