專任教師

姚少凌 (Chao-Ling Yao) 副教授

聯絡方式:

研究室:93A16
分機號碼:(06)-2757575轉62654
E-mail:yao8@gs.ncku.edu.tw
研究專長:
細胞工程、幹細胞技術、組織工程、生化與生醫工程、轉譯醫學、細胞免疫治療


NCKU Research: https://researchoutput.ncku.edu.tw/en/persons/chao-ling-yao

個人CV :

學歷

清華大學化工系 博士 (2004) / 碩士 (2000) / 學士 (1998)

經歷

2009.08-2021.07    元智大學 化學工程與材料科學學系  助理教授/副教授/教授

2010.08-2021.07    元智大學 生物科技與工程研究所  合聘助理教授/副教授/教授

2017.01-Present    中華民國基因檢測技術暨幹細胞研究發展協會  秘書長

2014.11-Present     財團法人竹清化工文教基金會  副董事長/董事/監事

2014.11-Present     財團法人李昭仁教授生醫工程發展基金會  副董事長/董事/監事

2012.03-Present     行政院衛生福利部桃園醫院 醫學倫理及人體試驗委員會(IRB)  委員

2004.10-2009.07    財團法人食品工業發展研究所 / 生物資源保存及研發中心  研究員

 

專業領域

細胞工程, 幹細胞技術, 組織工程,  生化與生醫工程, 轉譯醫學, 細胞免疫治療

開授課程

有機化學(一), 有機化學(二), 有機化學實驗(一), 有機化學實驗(二), 化工概論, 專題討論

教師著作

Journal Paper and Book Chapter:

  1. Jiang DL, Yao CL, Hu NJ*, Liu YC*., “Construction of a tandem repeat peptide sequence with pepsin cutting sites to produce recombinant α-melanocyte stimulating hormone.” Molecules 2021;26;6207.
  2. Yao CL, Huang P, Liu TC, Lin YK, Chen CY, Lai YT, Chin TY, Tseng TY, Hsu YC*., “CCL2 associated with CD38 expression during ex vivo expansion in human cord blood-derived hematopoietic stem cells.” Aging-US 2021;13(15):19878-19893.
  3. Mohamed MS, Elbadry MI, Yao CL*., “Engineered stem cells combine stem cell and gene therapy approaches to move intestine therapy from bench to bed.” The Intestine 2021;2:59-74. (ISBN: 978-0-12-821269-1)
  4. Mohamed MS, Elbadry MI, Yao CL*., “Stem-cell therapy with bone marrow (hematopoietic) stem cells for intestinal diseases.” The Intestine 2021;2:131-145. (ISBN: 978-0-12-821269-1)
  5. Lin KH, Chiang JC, Chen WM, Ho YH, Yao CL, Lee H*., “Transcriptional regulation of lysophosphatidic acid receptors 2 and 3 regulates myeloid commitment of hematopoietic stem cells.” American Journal of Physiology-Cell Physiology 2021;320(4):C509-C519.
  6. Chiang JC, Chen WM, Lin KH, Hsia K, Ho YH, Lin YC, Shen TL, Lu JH, Chen SK, Yao CL, Chen BPC, Lee H*., “Lysophosphatidic acid receptors 2 and 3 regulate erythropoiesis at different hematopoietic stages.” Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids 2021;1866(1):158818.
  7. Hsu CN, Chen YH, Yu CY, Wu SC, Lin SP, Yao CL*., “Glucocorticoid transiently upregulates mitochondrial biogenesis in the osteoblast.” Chinese Journal of Physiology 2020;63(6):286-293.
  8. Lin HD, Yao CL, Ou WJ, Luo YH, Chen SC*., “4-Aminobiphenyl suppresses homologous recombination repair by a reactive oxygen species-dependent p53/miR-513a-5p/p53 loop.” Toxicology 2020;444:152580.
  9. Lin HD, Wang FZ, Lee CY, Nien CY, Tseng YK, Yao CL, Chen SC*., “4-Aminobiphenyl inhibits the DNA homologous recombination repair in human liver cells: The role of miR-630 in downregulating RAD18 and MCM8.” Toxicology 2020;440:152441.
  10. Yao CL*, Lin CC, Chu IM, Lai YT., “Develop a surfactant-containing process to improved removal efficiency of phenol and control the molecular weight of synthetic phenolic polymer using horseradish peroxidase in aqueous system.” Applied Biochemistry and Biotechnology 2020;191(1):45-58.
  11. Lin KH, Chiang JC, Ho YH, Yao CL, Lee H*., “Lysophosphatidic acid and hematopoiesis: From microenvironmental effects to intracellular signaling.” International Journal of Molecular Sciences 2020;21(6):2015.
  12. Liu MY, Hua WK, Chiou YY, Chen CJ, Yao CL, Lai YT, Lin CH, Lin WJ*., “Calcium-dependent methylation by PRMT1 promotes erythroid differentiation through the p38α MAPK pathway.” FEBS Letters 2020;594(2):301-316.
  13. Yao CL*, Chi YW, Kao TT, Liu YL, Chiou TJ, Lai YT., “Effects of specific pathogen-free porcine platelet-rich plasma activated by the novel activator ectoine on cell proliferation and biological function.” Biochemical Engineering Journal 2020;153:107424.
  14. Lin CH, Lu JH*, Hsia K, Lee H, Yao CL, Ma H., “The antithrombotic function of sphingosine-1-phosphate on human adipose stem cell -recellularized tissue engineered vascular graft in virto.” International Journal of Molecular Sciences 2019;20(20):5218.
  15. Hsu SC, Lu LC, Chan KY, Huang CH, Cheng SL, Chan YS, Yang YS, Lai YT, Yao CL*., “Large-scale production and directed induction of functional dendritic cells ex vivo from serum-free expanded human hematopoietic stem cells.” Cytotherapy 2019;21(7):753-766.
  16. Hsia K, Lin CH, Lee H, Chen WM, Yao CL, Chen CC, Ma H, Wang SJ, Lu JH*., “Sphingosine-1-phosphate in endothelial cell recellularization improves patency and endothelialization of decellularized vascular grafts in vivo.” International Journal of Molecular Sciences 2019;20(7):1641.
  17. Juang LJ, Gao XY, Mai ST, Lee CH, Lee MC, Yao CL*., “Safety assessment, biological effects, and mechanisms of Myrica rubra fruit extract for anti-melanogenesis, anti-oxidation, and free radical scavenging abilities on melanoma cells.” Journal of Cosmetic Dermatology 2019;18(1):322-332.
  18. Chen Y, Yang XQ, Tseng BY, Tsai YH, Tseng SH, Lee CH, Yao CL*., “Deferoxamine preconditioning activated hypoxia-inducible factor-1α and MyD88-dependent Toll-like receptor 4 signaling in intestinal stem cells” Journal of Pediatric Surgery 2018;53(11):2349-2356.
  19. Juang RS, Chien CC, Yao CL, Yu SH, Sun AC*., “Preparation of magnetically recoverable mesoporous silica nanocomposites for effective adsorption of urea in simulated serum.” Journal of the Taiwan Institute of Chemical Engineers 2018;91:22-31.
  20. Yao CL*, Chen JH, Lee CH., “Effects of various monomers and micro-structure of polyhydroxyalkanoates on the behavior of endothelial progenitor cells and endothelial cells for vascular tissue engineering.” Journal of Polymer Research 2018;25(3):187.
  21. Chen Y, Tseng SH, Yao CL, Li C, Tsai YH*., “Distinct effects of growth hormone and glutamine on activation of intestinal stem cells” Journal of Parenteral and Enteral Nutrition 2018;42(3):642-651.
  22. Chen Y, Lee CH, Tseng BY, Tsai YH, Tsai HW, Yao CL*, Tseng SH*., “AZD8055 exerts antitumor effects on colon cancer cells by inhibiting mTOR and cell-cycle progression” Anticancer Research 2018;38(3):1445-1454.
  23. Lin KH, Li MW, Chang YC, Lin YN, Ho YH, Weng WC, Huang CJ, Chang BE, Yao CL*, Lee H*., “Activation of lysophosphatidic acid receptor 3 inhibits megakaryopoiesis in human hematopoietic stem cells and zebrafish.” Stem Cells and Development 2018;27(3):216-224.
  24. Kao CM, Wei SF, Chen SC, Yao CL, Ma C, Chien CC*., “Biotransformation of trinitrotoluene by Citrobacter sp. YC4 and evaluation of its cyto-toxicological effects.” FEMS Microbiology Letters 2018;365(1):fnx256.
  25. Tang BC, Yao CL, Xieh KY, Hong SG*., “Improvement of physical properties of poly(glycerol sebacate) by copolymerization with polyhydroxybutyrate-diols.” Journal of Polymer Research 2017;24(12):215.
  26. Lin YK, Sharma R, Ma H, Chen WS, Yao CL*., “In situ polymerizable hydrogel incorporated with specific pathogen-free porcine platelet-rich plasma for the reconstruction of the corneal endothelium.” Journal of the Taiwan Institute of Chemical Engineers 2017;78:65-74.
  27. Liao HY, Kao CM, Yao CL, Chiu PW, Yao CC, Chen SC*., “2,4,6-Trinitrotoluene induces apoptosis via ROS-regulated mitochondrial dysfunction and endoplasmic reticulum stress in HepG2 and Hep3B Cells.” Scientific Reports 2017;7:8148.
  28. Hsia K, Yang MJ, Chen W H, Yao CL, Lin CH, Loong CC, Huang YL, Lin YT, Lander AD, Lee H*, Lu JH*., “Sphingosine-1-phosphate improves endothelialization with reduction of thrombosis in recellularized human umbilical vein graft by inhibiting syndecan-1 shedding in vitro.” Acta Biomaterialia 2017;51:341-350.
  29. Cheng SL, Lin CH, Yao CL*., “Mesenchymal stem cell administration in patients with chronic obstructive pulmonary disease: state of the science.” Stem Cells International 2017;2017:8916570.
  30. Lin YK, Sugiri F, Chiu YH, Ma H, Lee CH, Yao CL*., “Industrial-scale processing of activated platelet-rich plasma from specific pathogen-free pigs and its effect on promoting human hair follicle dermal papilla cell cultivation.” Journal of the Taiwan Institute of Chemical Engineers 2017;71:28-37.
  31. Hsia K1, Yao CL1, Chen WM, Chen JH, Lee H*, Lu JH*., “Scaffolds and cell-based tissue engineering for blood vessel therapy.” Cells Tissues Organs 2015-16;202(5-6):281-295.
  32. Lee CH, Chen Y, Cheng CF, Yao CL, Wu JC, Yin WY, Chen JH*., “Incidence of and risk factors for pediatric metachronous contralateral inguinal hernia: analysis of a 17-year nationwide database in Taiwan.” PLoS One 2016;11(9):e0163278.
  33. Kao CM, Lin BH, Chen SC, Wei SF, Chen CC, Yao CL, Chien CC*., “Biodegradation of trinitrotoluene (TNT) by indigenous microorganisms from TNT-contaminated soil, and their application in TNT bioremediation.” Bioremediation Journal 2016;20(3):165-173.
  34. Lin KH, Ho YH, Chiang JC, Li MW, Lin SH, Chen WM, Chiang CL, Lin YN, Yang YJ, Chen CN, Lu JH, Huang CJ, Tigyi G, Yao CL*, Lee H*., “Pharmacological activation of lysophosphatidic acid receptors regulates erythropoiesis.” Scientific Reports 2016;6:27050.
  35. Yao CL*, Liao CJ, Liang SM., “Characterization and implantation of a novel foamy type of collagen into SD rats to regenerate tissue by slowing down the collagen degradation rate.” International Journal of Polymeric Materials and Polymeric Biomaterials 2016;65(10):508-515.  
  36. Sin MC, Sun YM*, Yao CL, Chou CJ, Tseng HW, Zheng J, Chang Y*., “PEGylated poly(3-hydroxybutyrate) scaffold for hydration-driven cell infiltration, neo-tissue ingrowth, and osteogenic potential.” International Journal of Polymeric Materials and Polymeric Biomaterials 2015;64(16):865-878.
  37. Mohamed MS, Chen Y, Yao CL*., “Intestinal stem cells and stem cell-based therapy for intestinal diseases.” Cytotechnology 2015;67(2):177-189. 
  38. Ho YH, Yao CL, Lin KH, Hou FH, Chen WM, Chiang CL, Lin YN, Li MW, Lin SH, Yang YJ, Lin CC, Lu JH*, Tigyi G*, Lee H*., “Opposing regulation of megakaryopoiesis by LPA receptors 2 and 3 in K562 human erythroleukemia cells.” Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids 2015;1851(2):172-183.
  39. Hsu SH*, Ho TT, Huang NC, Yao CL, Peng LH, Dai NT., “Substrate-dependent modulation of 3D spheroid morphology self-assembled in mesenchymal stem cell-endothelial progenitor cell coculture.” Biomaterials 2014;35(26):7295-7307.
  40. Mohamed MS, Chen Y, Yao CL*., “A serum-free medium developed for expansion of murine intestinal stem cells in vitro.” Biotechnology Journal 2014;9(7):962-970.
  41. Yao CL*, Hsu SC, Hwang SM, Lee WC, Chiou TJ., “A stromal-free, serum-free system to expand ex vivo hematopoietic stem cells from mobilized peripheral blood of patients with hematological malignancies and healthy donors.” Cytotherapy 2013;15(9):1126-1135.
  42. Yao CL*, Lin YM, Mohamed MS, Chen JH., “Inhibitory effect of ectoine on melanogenesis in B16-F0 and A2058 melanoma cell lines.” Biochemical Engineering Journal 2013;78:163-169.
  43. Chen WC, Yu WJ, Chang CC, Chang JS, Huang SH, Chang CH, Chen SY, Chien CC, Yao CL, Chen WM, Wei YH., “Enhancing production of prodigiosin from Serratia marcescens C3 by statistical experimental design and porous carrier addition strategy.” Biochemical Engineering Journal 2013;78:93-100.
  44. Chen WC, Wei YH, Chu IM, Yao CL*., “Effect of the chondroitin sulfate C on the in vitro and in vivo chondrogenesis of mesenchymal stem cells in crosslinked type II collagen scaffolds.” Journal of Tissue Engineering and Regenerative Medicine 2013;7(8):665-672.
  45. Hua WK, Chang YI, Yao CL, Hwang SM, Chang CY, Lin WJ., “Protein arginine methyltransferase 1 interacts with and activates p38α to facilitate erythroid differentiation.” PLoS One 2013;8(3):e56715.
  46. Hsu SC, Wang LT, Yao CL, Lai HY, Chan KY, Liu BS, Chong P, Lee OK, Chen HW., “Mesenchymal stem cells promote neutrophil activation by inducing IL-17 production in CD4+ CD45RO+ T cells.” Immunobiology 2013;218(1):90-95.
  47. Yao CL, Hwang SM., “Immortalization of human mesenchymal stromal cells with telomerase and red fluorescence protein expression.” Methods in Molecular Biology 2012;879:471-478 (ISBN: 978-1-61779-814-6).
  48. Chiang CL, Chen SSA, Lee SJ, Tsao KC, Chu PL, Wen CH, Hwang SM, Yao CL*, Lee H*., “Lysophosphatidic acid induces erythropoiesis through activating lysophosphatidic acid receptor 3.” Stem Cells 2011;29:1763-1773.
  49. Yen CL, Chen WJ, Lo ST, Chen KT, Yao CL, Lee TW, Lo JM., “Biodistribution of Tc-99m(I)-tricarbonyl labeled trastuzumab as an imaging agent for breast cancer with HER2 overexpression in an animal model.” Annals of Nuclear Medicine and Molecular Imaging 2011;24(3):119-122.
  50. Chen WC, Wei YH, Huang JB, Chu IM, Yao CL*., “Biological effects of oligosaccharide chondroitin sulfate C on human articular chondrocytes.” Biomedical Engineering-Applications Basis Communications 2011;23:245-252.
  51. Chen WC, Yao CL, Chu IM, Wei YH., “Compare the effects of chondrogenesis by culture of human mesenchymal stem cells with various type of the chondroitin sulfate C.” Journal of Bioscience and Bioengineering 2011;111:226-231.
  52. Chen WC, Yao CL, Wei YH, Chu IM., “Evaluating osteochondral defect repair potential of autologous rabbit bone marrow cells on type II collagen scaffold.” Cytotechnology 2011;63:13-23.
  53. Hung CJ1, Yao CL1, Cheng FC, Wu ML, Wang TH, Hwang SM., “Establishment of immortalized mesenchymal stromal cells with red fluorescence protein expression for in vivo transplantation and tracing in the rat model with traumatic brain injury.” Cytotherapy 2010;12:455-465.
  54. Chang YI, Hua WK, Yao CL, Hwang SM, Hung YC, Kuan CJ, Leou JS, Lin WJ., “Protein-arginine methyltransferase 1 suppresses megakaryocytic differentiation via modulation of the p38 MAPK pathway in K562 cells.” Journal of Biological Chemistry 2010;285:20595-20606.
  55. Chen TW, Hwang SM, Chu IM, Hsu SC, Hsieh TB, Yao CL*, “Characterization and transplantation of induced megakaryocytes from hematopoietic stem cells for rapid platelet recovery by a two-step serum-free procedure.” Experimental Hematology 2009;37:1330-1339.
  56. Chen TW1,
    Yao CL1, Chu IM, Chuang TL, Hsieh TB, Hwang SM., “Large generation of megakaryocytes from serum-free expanded human CD34+ Cells.” Biochemical and Biophysical Research Communications 2009;378:112-117.
  57. Kao IT1, Yao CL1, Chang YJ, Hsieh TB, Hwang SM., “Chondrogenic differentiation of human mesenchymal stem cells from umbilical cord blood in thermoreversible gelation polymer.” Chinese Journal of Physiology 2008;51:252-258.
  58. Chang CL, Lin ME, Hsu HY, Yao CL, Hwang SM, Pan CY, Hsu CY, Lee H., “Lysophosphatidic acid-induced interleukin-1β expression is mediated through Gi/Rho and the generation of reactive oxygen species in macrophages.” Journal of Biomedical Science 2008;15:357-363.
  59. Yeh HJ, Yao CL, Chen HI, Cheng HC, Hwang SM., “Cryopreservation of human limbal stem cells ex vivo expanded on amniotic membrane.” Cornea 2008; 27:327-333. (Troutman Prize, The Cornea Society 2008)
  60. Yao CL*, Hwang SM., “Ex vivo expansion of hematopoietic stem cells from human cord blood in serum-free conditions.” Methods in Molecular Biology 2007;407:165-175. (ISBN: 978-1-58829-744-0).
  61. Kao IT1, Yao CL1, Kong ZL, Wu ML, Chuang TL, Hwang SM., “Generation of natural killer cells from serum-free expanded human umbilical cord blood CD34+ cells.” Stem Cells and Development 2007;16:1043-1052.
  62. Yao CL, Feng YH, Lin XZ, Chu IM, Hsieh TB, Hwang SM., “Characterization of serum-free ex vivo-expanded hematopoietic stem cells derived from human umbilical cord blood CD133+ cells.” Stem Cells and Development 2006;15:70-80.
  63. Yao CL, Hwang SM., “Systematic strategy approach in medium design.” Experimental Hematology 2005;33:1273-1274.
  64. Yao CL, Chu IM, Hsieh TB, Hwang SM., “A systematic strategy to optimize ex vivo expansion medium for human hematopoietic stem cells derived from umbilical cord blood mononuclear cells.” Experimental Hematology 2004;32:720-727.
  65. Yao CL, Liu CH, Chu IM, Hsieh TB, Hwang SM., “Factorial designs combined with the steepest ascent method to optimize serum-free media for ex vivo expansion of human hematopoietic progenitor cells.” Enzyme and Microbial Technology 2003;33:343-352.
  66. 姚少凌., “臍帶血血漿加值應用-造血幹細胞增殖應用技術” 生物科技特刊, 食品資訊雜誌社 2017-06.

  67. 姚少凌, 黃效民., “活躍的造血幹細胞” 科學發展月刊 2007;414:12-17.

 

Patent:

  1. Hwang SM, Liu CH, Yao CL, Chu IM, Hsieh TB., Optimizing culture medium for CD34+ hematopoietic cell expansion. (US 8762074 B2, 2012/5/10-2032/5/10)

  2. Hwang SM, Yao CL, Hsieh TB, Chu IM., Stroma-free, serum-free, and chemically defined medium and method for ex vivo mononuclear cell expansion using the same. (US 7723106 B2, 2010/5/25-2027/1/28)

  3. Chen Y, Yao CL., 體外小腸幹細胞培養基及培養方法, Culture media and culture method for intestinal stem cells in vitro. (中華民國發明專利I522467, 2016/02/21-2034/05/07。中國專利CN 105087459A)

  4. 姚少凌, 魏毓宏., 具有相容性溶質之冷凍細胞保存組合物於人類造血幹細胞之應用. (中華民國發明專利 I681955, 2020/01/11-2037/03/06)

  5. 姚少凌., 不具胎牛血清之細胞冷凍保存組合物. (中華民國發明專利 I640630, 2018/11/11-2037/11/20)

  6. 姚少凌, 錡永文., 促進富含血小板血漿釋放生長因子之方法. (中華民國發明專利 I723482, 2021/04/01-2039/07/30)

 

指導學生

博一:曾琮祐

碩一:林于哲, 陳劭宇, 蔡秉諭

碩零:許馨云, 林意儒, 黃烽展, 林柏宇

研究

幹細胞生物學與工程, 細胞培養與細胞工程, 動物移植模型, 細胞治療與組織工程, 轉譯醫學與再生醫學, (幹)細胞篩選平台, 生化工程與生醫工程