Flavonoids exhibit substantial medicinal, antioxidative, anti-inflammatory, and cancer-prevention properties and have found extensive applications in traditional medicine, agriculture, food, cosmetics, and manufacturing for centuries. The glycosylation process, involving the addition of sugar to proteins, lipids, or other organic molecules, plays a pivotal role in numerous biological processes. Glycosylation enhances the solubility of molecules in aqueous solutions, rendering them more suitable for diverse applications while improving their bioavailability and absorption in biological systems. Some glycosylated flavonoids naturally occur in plants and are incorporated into health-promoting foods. To advance the development of compounds with potential health benefits, we devised the Predicted Data Mining Approach (PDMA). This method integrates in vitro biotransformation techniques with insights from traditional medicine, aiming to generate novel compounds for human health enhancement.