The balance-charge with high polarity chemical structure, zwitterion, such as sulfobetaine (SB) and carboxylbetaine (CB), perform strong hydrophilicity and biofouling resistance. The surface grafting of epoxlated zwitterionic copolymer, ploy(glycidyl methacrylate-co-sulfobetaine methacrylate) (poly(GMA-co-SBMA)) is a simple, efficient and robust antifouling modification for versatile materials including ceramic, metal, and plastics. Based the diversity of poly(GMA-co-SBMA), it could be applied to different types of devices, such as the modification of silicone, nanoparticles, biosensor chips, and textile auxiliaries. The grafting principle of poly(GMA-co-SBMA) modified surface is based on the base-induced ring-opening reaction between epoxy groups and hydroxyl groups. The universal surface modification procedure was developed and performed from an optimized sequence of ultra-violet ozone pretreatment and trimethylamine-catalyzed zwitterionization on a selective case of versatile surfaces including silicon wafer, ceramic glass, titanium, steel, and polystyrene.